Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Low Doses of Celecoxib Attenuate Gut Barrier Failure During Experimental Peritonitis

doi: 10.1038/labinvest.2013.119

Figure Lengend Snippet: (a) H&E-stained sections of ileum from mice 16 h after injection with NS, injection with 40 mg/kg LPS, sham operation, or CLP. Note abundant epithelial sloughing in animals injected with LPS or subjected to CLP. Data are representative of at least 9 animals in each group. (b) Transepithelial passage of FITC-dextran and bacteria during peritonitis. Mice were gavaged with FITC-dextran and E. coli 35354T prior to indicated treatments. Serum concentrations of FITC-dextran (top) and counts of viable E. coli in combined mesenteric lymph nodes and spleens (bottom) were determined 16 h post treatment. *, Significant differences (p ≤ 0.01, n ≥ 12 in each group). (c) Localization of JAM-A (red) in ileal epithelium 16 h after indicated treatments. Note increased re-distribution of JAM-A from borders to intracellular space of enterocytes in LPS and CLP samples. (d) Localization of ZO-1 (red) in ileal epithelium 16 h after indicated treatments. Note increased redistribution of ZO-1 from TJ to intracellular space of enterocytes in LPS and CLP samples. DAPI-stained nuclei appear in blue. Bar=50 μm. All images are representative of at least 3 animals in each group.

Article Snippet: Commensal E. coli strain 35354T was obtained from ATCC (Manassas, VA) and transformed with the pUC18 plasmid to confer ampicillin resistance.

Techniques: Staining, Injection, Bacteria

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Low Doses of Celecoxib Attenuate Gut Barrier Failure During Experimental Peritonitis

doi: 10.1038/labinvest.2013.119

Figure Lengend Snippet: Mice were gavaged with the test mixture of FITC-dextran and E. coli prior to experiments. Celecoxib at indicated doses (mg/kg) or vehicle were added to the test mixture. (a) Serum FITC-dextran levels 16 h after injection with NS or LPS, with or without various doses of Celecoxib, as indicated. (b) Serum FITC-dextran levels 16 h after sham operation or CLP, with or without various doses of Celecoxib, as indicated. (c) E. coli counts in lymphatic organs 16 h after injection with NS or LPS, with or without various doses of Celecoxib, as indicated. (d) E. coli counts in lymphatic organs 16 h after sham operation or CLP, with or without Celecoxib, as indicated. (e) E. coli counts in liver 16 h after indicated treatments, with or without 0.5 mg/kg Celecoxib, as indicated. Data are average ± SEM, n≥12 in each group. Significant differences: *, p ≤0.01 vs. LPS or CLP alone; **, p≤0.02 vs. LPS or CLP alone; #, p≤0.02 vs. NS or sham.

Article Snippet: Commensal E. coli strain 35354T was obtained from ATCC (Manassas, VA) and transformed with the pUC18 plasmid to confer ampicillin resistance.

Techniques: Injection

Journal: Antibiotics

Article Title: NRX-101 (D-Cycloserine + Lurasidone) Is Active against Drug-Resistant Urinary Pathogens In Vitro

doi: 10.3390/antibiotics13040308

Figure Lengend Snippet: Strains tested and their antimicrobial resistance profiles.

Article Snippet: Escherichia coli ATCC BAA-2469 , Carbepenem-resistant (imipenem and ertapenem).

Techniques:

Journal: Italian Journal of Food Safety

Article Title: Behaviour of Listeria Monocytogenes and Escherichia Coli O157:H7 During the Cheese Making of Traditional Raw-Milk Cheeses from Italian Alps

doi: 10.4081/ijfs.2015.4585

Figure Lengend Snippet: Logc concentration of Listeria monocytogenes (white bars) and Escherichia coli O157:H7 (grey bars), throughout the cheese making and ripening of short ripened cheese. Values are obtained from the average and standard deviations of three samples at each sampling time.

Article Snippet: L. monocytogenes ATCC ® 19115 and two wild strains (isolated from cheeses; BVR; www.ibvr.org ) and E. coli O157:H7 ATCC ® 35150 and two wild strains (isolated from milk; BVR; www.ibvr.org ) were used in the challenge test.

Techniques: Concentration Assay, Sampling

Journal: Italian Journal of Food Safety

Article Title: Behaviour of Listeria Monocytogenes and Escherichia Coli O157:H7 During the Cheese Making of Traditional Raw-Milk Cheeses from Italian Alps

doi: 10.4081/ijfs.2015.4585

Figure Lengend Snippet: Logc concentration of Listeria monocytogenes (white bars) and Escherichia coli O157:H7 (grey bars), throughout the cheese making and ripening of long ripened cheese. Values are obtained from the average and standard deviations of two samples at each sampling time.

Article Snippet: L. monocytogenes ATCC ® 19115 and two wild strains (isolated from cheeses; BVR; www.ibvr.org ) and E. coli O157:H7 ATCC ® 35150 and two wild strains (isolated from milk; BVR; www.ibvr.org ) were used in the challenge test.

Techniques: Concentration Assay, Sampling

Journal: Frontiers in Pharmacology

Article Title: Interference in Bacterial Quorum Sensing: A Biopharmaceutical Perspective

doi: 10.3389/fphar.2018.00203

Figure Lengend Snippet: C. elegans survival rate upon quorum quenching of several virulent bacteria.

Article Snippet: Combination of the acylase with α-amylase from Bacillus amyloliquefaciens delayed biofilm development of both P. aeruginosa ATCC 10145 and E. coli ATCC 25922 for up to 7 days in an in vivo rabbit model (Ivanova et al., ).

Techniques: Bacteria, Concentration Assay, Infection, Isolation

Journal: Clinical Pharmacology : Advances and Applications

Article Title: Possible involvement of ROS generation in vorinostat pretreatment induced enhancement of the antibacterial activity of ciprofloxacin

doi: 10.2147/CPAA.S148448

Figure Lengend Snippet: Ciprofloxacin-induced antibacterial action on Escherichia coli cells is preceded by a time-dependent reactive oxygen species (ROS) generation. Notes: ( A ) Mean fluorescence intensity (MFI) was shown as the ratio of geometric mean fluorescence intensity of the test sample and the corresponding control. Data shown are representative of three individual experiments. ( B ) Pretreatment of E. coli cells with vorinostat (100 µM) for 16 hours enhanced ciprofloxacin-induced ROS generation; 2′,7′-dichlorofluorescein diacetate (DCF-DA; 10 µM) was added for the last 30 minutes of incubation. The intensity of DCF-DA fluorescence was determined using flow cytometry, with an excitation wavelength of 480 nm and an emission wavelength of 530 nm. Data shown are representative of three individual experiments. *Significant difference from the control, and ciprofloxacin-only treated groups (one-way ANOVA followed by Tukey’s post hoc test, p <0.05 in each case).

Article Snippet: Activity of the ciprofloxacin–vorinostat combination were investigated across a panel of bacterial reference strains that included Streptococcus pneumoniae ATCC 25923, methicillin-resistant Staphylococcus aureus (MRSA) ATCC 43300, Klebsiella pneumoniae ATCC 13883, Proteus mirabilis ATCC 12459, Acinetobacter baumannii ATCC 17978, Staphylococcus epidermidis ATCC 12228, Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC29213, and Escherichia coli ATCC 35218.

Techniques: Fluorescence, Control, Incubation, Flow Cytometry

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Exploitation of E. coli for the production of penicillin G amidase: a tool for the synthesis of semisynthetic β-lactam antibiotics

doi: 10.1186/s43141-021-00263-7

Figure Lengend Snippet: Production kinetics of PGA producing wild type strains

Article Snippet: A four times more productive mutant than the parent strain has been produced by chemical mutagenesis of E. coli ATCC 11105 with NTG [ ].

Techniques: Incubation, Purification

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Exploitation of E. coli for the production of penicillin G amidase: a tool for the synthesis of semisynthetic β-lactam antibiotics

doi: 10.1186/s43141-021-00263-7

Figure Lengend Snippet: Production kinetics of PGA producing recombinant strains

Article Snippet: A four times more productive mutant than the parent strain has been produced by chemical mutagenesis of E. coli ATCC 11105 with NTG [ ].

Techniques: Recombinant, Incubation

Journal: bioRxiv

Article Title: Comparison of transcriptional responses and metabolic alterations in three multidrug resistant model microorganisms, Staphylococcus aureus ATCC BAA-39, Escherichia coli ATCC BAA-196 and Acinetobacter baumannii ATCC BAA-1790, on exposure to iodine-containing nano-micelle drug FS-1

doi: 10.1101/2020.09.01.278945

Figure Lengend Snippet: A) E. coli BAA-196 (EC-BAA-196 Lag/Log); B) S. aureus BAA-39 (SA-BAA-39 Lag/Log); and C) A. baumannii BAA-1790 (AB-BAA-1790 Lag/Log). Circles represent protein coding genes (CDS) plotted according to their negative and positive log 2 (fold-change) values calculated in the Lag-experiment (axis X) and Log-experiment (axis Y). The outermost regulated genes are labelled by their names or locus tag numbers. Thin vertical and horizontal lines within the plots separate genes with 2-fold or higher regulation and split the plots into sectors of genes of different categories depending on their coregulation. Numbers of CDS falling to different sectors are shown. Up- and down-coregulated genes, oppositely regulated genes and the genes regulated only in one experiment are depicted by different colours. Statistical reliability of the fold change predictions is depicted by sizes of circles as explained in the legend on the bottom part of the figure. Estimated Pearson correlation coefficients are given on the top of each plot.

Article Snippet: Genes regulated in E. coli ATCC BAA-196 in Lag and Log growth phases by 5 min exposure to FS-1.

Techniques:

Journal: bioRxiv

Article Title: Comparison of transcriptional responses and metabolic alterations in three multidrug resistant model microorganisms, Staphylococcus aureus ATCC BAA-39, Escherichia coli ATCC BAA-196 and Acinetobacter baumannii ATCC BAA-1790, on exposure to iodine-containing nano-micelle drug FS-1

doi: 10.1101/2020.09.01.278945

Figure Lengend Snippet: A) E. coli BAA-196 and S. aureus BAA-39 (EC-BAA-196 / SA-BAA-39); B) E. coli BAA-196 and A. baumannii BAA-1790 (EC-BAA-196 /AB_BAA-1790); and C) S. aureus BAA-39 and A. baumannii BAA-1790 (SA-BAA-39 / AB-BAA-1790). Circles represent protein coding genes (CDS) plotted according to their negative and positive log 2 (fold-change) values calculated in the Lag-experiments for different microorganisms shown along axes X and Y. The outermost regulated genes are labelled by their names. Thin vertical and horizontal lines within the plots separate genes with 2-fold or higher regulation and split the plots to sectors of genes of different categories depending on their coregulation. Numbers of CDS falling to different sectors are shown. Up- and down-coregulated genes and oppositely regulated genes are depicted by different colours. Statistical reliability of the fold change predictions is depicted by sizes of the circles as explained in the legend in the bottom part of the figure. Estimated Pearson correlation coefficients are given on the top of each plot.

Article Snippet: Genes regulated in E. coli ATCC BAA-196 in Lag and Log growth phases by 5 min exposure to FS-1.

Techniques:

Journal: Biophysical Journal

Article Title: The In Situ Tryptophan Analogue Probes the Conformational Dynamics in Asparaginase Isozymes

doi: 10.1016/j.bpj.2016.03.029

Figure Lengend Snippet: (a) Structures of Trp and the Trp analog probes. (b and c) Comparison of the crystallography structures of E. coli (b) AnsA (PDB: 2HIM) and (c) AnsB (PDB: 3ECA) shows the local Trp environment with the relevant residues colored blue for polar and red for nonpolar amino acids, also highlighting the substrate binding-pocket residues S60 and D92 for AnsA and S58 and D90 for AnsB. The other relevant residues are represented by yellow sticks with blue and red indicating the nitrogen and oxygen positions, respectively, and the relevant water molecules appear as pink spheres. To see this figure in color, go online.

Article Snippet: A Trp auxotrophic E. coli strain (ATCC 23231) was utilized for protein expression.

Techniques: Comparison, Binding Assay